CellProfiler, a software package for high-throughput image analysis by interactive construction of workflow.Bitplane - producers of image processing software with ImageJ compatibility.Bio7 - an Integrated Development Environment for Ecological Modeling, Scientific Image Analysis and Statistical Analysis embedding ImageJ as an Eclipse view.Both versions are still available but – in contrast to NIH Image – closed-source. A Windows version – ported by Scion Corporation (now defunct), so-called Scion Image for Windows – was also developed. Further development of this code continues in the form of Image SXM, a variant tailored for physical research of scanning microscope images. History īefore the release of ImageJ in 1997, a similar freeware image analysis program known as NIH Image had been developed in Object Pascal for Macintosh computers running pre-OS X operating systems. The program supports any number of images simultaneously, limited only by available memory. It does geometric transformations such as scaling, rotation, and flips. It supports standard image processing functions such as logical and arithmetical operations between images, contrast manipulation, convolution, Fourier analysis, sharpening, smoothing, edge detection, and median filtering. It can create density histograms and line profile plots. ImageJ can calculate area and pixel value statistics of user-defined selections and intensity-thresholded objects. ImageJ supports image stacks, a series of images that share a single window, and it is multithreaded, so time-consuming operations can be performed in parallel on multi-CPU hardware. It can read many image file formats, including TIFF, PNG, GIF, JPEG, BMP, DICOM, and FITS, as well as raw formats. ImageJ can display, edit, analyze, process, save, and print 8-bit color and grayscale, 16-bit integer, and 32-bit floating point images. ImageJ's plugin architecture and built-in development environment has made it a popular platform for teaching image processing. User-written plugins make it possible to solve many image processing and analysis problems, from three-dimensional live-cell imaging to radiological image processing, multiple imaging system data comparisons to automated hematology systems. Custom acquisition, analysis and processing plugins can be developed using ImageJ's built-in editor and a Java compiler. ImageJ was designed with an open architecture that provides extensibility via Java plugins and recordable macros. Its first version, ImageJ 1.x, is developed in the public domain, while ImageJ2 and the related projects SciJava, ImgLib2, and SCIFIO are licensed with a permissive BSD-2 license. ImageJ is a Java-based image processing program developed at the National Institutes of Health and the Laboratory for Optical and Computational Instrumentation (LOCI, University of Wisconsin).
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